Journal: Clinical & Translational Immunology

Article Title: Human placenta-derived adherent cells induce tolerogenic immune responses

doi: 10.1038/cti.2014.5

Figure Lengend Snippet: PDAC cells display MSC-like characteristics. ( a ) PDAC cells from two donors show spindle-shaped fibroblast morphology under phase contrast microscope after 6 passages of culture expansion ex vivo . ( b ) Flow cytometry analysis of PDAC-cell phenotype. Top row: expression of MSC markers (CD73, CD90, CD105) and placenta marker (CD200). Bottom row: expression of hematopoietic cell surface markers. Dotted lines indicate isotype-matched control antibody staining. Numbers indicate percent positive cells. Results from one of the six independent experiments are shown. ( c ) Adipogenic (Oil red O staining), osteogenic (alizarin red staining) and chondrogenic (Alcian Blue and Sirius Red staining) differentiation of PDAC cells under different lineage differentiation culture conditions.

Article Snippet: For adipogenic and osteogenic differentiation, the PDAC cell expansion medium was changed to adipogenic (DMEM high glucose medium supplemented with 10% Fetal Calf Serum, 10 μg ml −1 insulin, 1 μ M dexmethasone, 0.1 m M indomethacin, 0.5 m M isobutylmethylxanthine and 100 IU ml −1 Pen-Strep) or osteogenic induction medium (Cambrex, East Rutherford, NJ, USA).

Techniques: Microscopy, Ex Vivo, Flow Cytometry, Expressing, Marker, Control, Staining

Journal: Animal Models and Experimental Medicine

Article Title: Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability

doi: 10.1002/ame2.12513

Figure Lengend Snippet: Bone morphogenetic protein 9 (BMP9) induces osteogenic differentiation of immortalized rabbit bone marrow mesenchymal stem cells (iRBMSCs) in vitro. (A) Alkaline phosphatase (ALP) staining (left) and quantification (right) of RBMSCs (scale = 200 μm, * p < 0.05, ** p < 0.01). (B) ALP staining (left) and quantification (right) of iRBMSCs (scale = 200 μm, ** p < 0.01). (C) Alizarin red staining (left) and quantification (right) of RBMSCs (scale = 200 μm, ** p < 0.01). (D) Alizarin red staining (left) and quantification (right) of iRBMSCs (scale = 200 μm, ** p < 0.01). (E) Expression levels of osteogenic genes in the BMP9‐induced iRBMSCs were determined using reverse transcription quantitative polymerase chain reaction (RT‐qPCR) on Days 3 and 7. All values are the mean ± SDs (* p < 0.05, ** p < 0.01).

Article Snippet: Dulbecco modified Eagle medium (DMEM) and α‐MEM (minimum essential medium) were purchased from Gibco (Massachusetts, USA); trypsin–EDTA, percoll cell isolation solution, alkaline phosphatase staining and quantitative kit, alizarin red S staining kit, Alcian blue staining kit, and MTT kit were all purchased from Solarbio (Beijing, China); osteogenic induction medium, lipogenic induction medium, and chondrogenic induction medium were all purchased from Procell (Wuhan, China).

Techniques: In Vitro, Staining, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

Journal: Animal Models and Experimental Medicine

Article Title: Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability

doi: 10.1002/ame2.12513

Figure Lengend Snippet: Preparation of GelMA hydrogels for use as osteogenic scaffolds in vivo. (A) Degradation of GelMA hydrogel at different concentrations for 14 days. (B) The activity of immortalized rabbit bone marrow mesenchymal stem cells (iRBMSCs) in 20%(w/v) GelMA hydrogel was detected using calcein/PI staining (live cells [green], dead cells [red]. Scale = 100 μm). (C) Alkaline phosphatase (ALP) staining (left) and alizarin red staining (right) of complexes consisting of GelMA hydrogel and BMP9‐induced iRBMSCs.

Article Snippet: Dulbecco modified Eagle medium (DMEM) and α‐MEM (minimum essential medium) were purchased from Gibco (Massachusetts, USA); trypsin–EDTA, percoll cell isolation solution, alkaline phosphatase staining and quantitative kit, alizarin red S staining kit, Alcian blue staining kit, and MTT kit were all purchased from Solarbio (Beijing, China); osteogenic induction medium, lipogenic induction medium, and chondrogenic induction medium were all purchased from Procell (Wuhan, China).

Techniques: In Vivo, Activity Assay, Staining

Journal: Animal Models and Experimental Medicine

Article Title: Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability

doi: 10.1002/ame2.12513

Figure Lengend Snippet: Bone morphogenetic protein 9 (BMP9) and GelMA hydrogels synergistically induce ectopic bone formation from immortalized rabbit bone marrow mesenchymal stem cells (iRBMSCs) in vivo. (A) Liquid GelMA (left) and solid GelMA hydrogel (right). (B) Schematic diagram of dorsal subcutaneous scaffold material implantation in athymic nude mice. (C) Micro‐computed tomography (μCT) software was used to scan ectopic bone specimens obtained 8 weeks after transplantation. (D–G) New born bone volume fraction (BV/TV), the number of trabeculae (Tb.N), the thickness of trabeculae (Tb.Sp), and trabecular separation (Tb.Th) of ectopic osteogenic specimens obtained by analysis with μCT software. All values are mean ± SD. * p < 0.05, ** p < 0.01. (H) H&E staining. (I) Masson staining (Scale bar = 200 μm, ▲, indicates newly formed bone. Enlarged image scale bar = 50 μm).

Article Snippet: Dulbecco modified Eagle medium (DMEM) and α‐MEM (minimum essential medium) were purchased from Gibco (Massachusetts, USA); trypsin–EDTA, percoll cell isolation solution, alkaline phosphatase staining and quantitative kit, alizarin red S staining kit, Alcian blue staining kit, and MTT kit were all purchased from Solarbio (Beijing, China); osteogenic induction medium, lipogenic induction medium, and chondrogenic induction medium were all purchased from Procell (Wuhan, China).

Techniques: In Vivo, Micro-CT, Software, Transplantation Assay, Staining