Journal: Clinical & Translational Immunology

Article Title: Human placenta-derived adherent cells induce tolerogenic immune responses

doi: 10.1038/cti.2014.5

Figure Lengend Snippet: PDAC cells display MSC-like characteristics. ( a ) PDAC cells from two donors show spindle-shaped fibroblast morphology under phase contrast microscope after 6 passages of culture expansion ex vivo . ( b ) Flow cytometry analysis of PDAC-cell phenotype. Top row: expression of MSC markers (CD73, CD90, CD105) and placenta marker (CD200). Bottom row: expression of hematopoietic cell surface markers. Dotted lines indicate isotype-matched control antibody staining. Numbers indicate percent positive cells. Results from one of the six independent experiments are shown. ( c ) Adipogenic (Oil red O staining), osteogenic (alizarin red staining) and chondrogenic (Alcian Blue and Sirius Red staining) differentiation of PDAC cells under different lineage differentiation culture conditions.

Article Snippet: For adipogenic and osteogenic differentiation, the PDAC cell expansion medium was changed to adipogenic (DMEM high glucose medium supplemented with 10% Fetal Calf Serum, 10 μg ml −1 insulin, 1 μ M dexmethasone, 0.1 m M indomethacin, 0.5 m M isobutylmethylxanthine and 100 IU ml −1 Pen-Strep) or osteogenic induction medium (Cambrex, East Rutherford, NJ, USA).

Techniques: Microscopy, Ex Vivo, Flow Cytometry, Expressing, Marker, Control, Staining

Journal: Bioengineering & Translational Medicine

Article Title: A simple and efficient strategy for cell‐based and cell‐free‐based therapies in acute liver failure: hUCMSCs bioartificial liver

doi: 10.1002/btm2.10552

Figure Lengend Snippet: hUCMSCs acquisition and identification, and large‐scale expansion in hollow fiber bioreactor. (a) Schematic diagram of acquisition and identification, and large‐scale expansion of hUCMSCs, which were first expanded to 10 7 –10 8 in the culture flask, then inoculated into the hollow fiber bioreactor until amplified to 10 9 –10 10 ; (b) hUCMSCs were isolated by the tissue block method; (c) P5‐generation hUCMSCs morphology under an optical inverted microscope. (d) hUCMSCs differentiated into fat and stained with Oil red O (×200). (e) Osteogenic differentiation of hUCMSCs stained with alizarin red (×200). (f) Cartilage differentiation of hUCMSCs stained with safranin O (×200). (g) Flow cytometry showed that hUCMSCs highly expressed CD73, CD90, and CD105 (both ≥ 95%) but did not express CD34, CD45, CD19, CD11b, or HLA‐DR (both ≤ 5%); (h) The results of live and dead staining of hUCMSCs in the bioreactor; (i) hUCMSCs in the bioreactor under a light microscope; (j) Hollow‐fiber superfine structure in the bioreactor. (k) Adhesion of hUCMSCs to hollow fiber in the bioreactor. (l–m) Changes in GLU, Lac, and PO 2 in the bioreactor during hUCMSCs culture in (l) Group B and (m) Group C. GLU, glucose; Lac, lactate; PO 2 , oxygen partial pressure. Scale bar 200 μm.

Article Snippet: According to the manufacturer's instructions, adipogenic induction medium (STEMCELL, Catalog #05412), osteogenic induction medium (STEMCELL, Catalog #05465), and chondrogenic induction medium (STEMCELL, Catalog #05455) were used to culture hUCMSCs.

Techniques: Amplification, Isolation, Blocking Assay, Inverted Microscopy, Staining, Flow Cytometry, Light Microscopy